Virus:Article Title: Bacterial metabolites induce cell wall remodeling, antifungal resistance, and immune recognition of commensal fungi.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc-hDectin-1a Invivogen Cat# fc-hdec1a-2 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21445 Alexa Fluor® 647 AffiniPure® Goat Anti-Human Serum IgA, α chain specific Jackson ImmunoResearch Cat# 109-605-011; RRID: AB_2337883 Hog1 antibody Santa Cruz Bioscience Cat# sc-165978; RRID: AB_2012916 IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody LICOR Bioscience Cat# 926-32210; RRID: AB_621842 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 9215; RRID: AB_331762 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Bacterial and virus strains Escherichia coli, MC1061 Provided by Sandkvist lab NCBI: txid1211845 Escherichia coli, MG1655 Blattner et al. 66 (Provided by Mobley lab) NCBI: txid511145 Escherichia coli, CFT073 Mobley et al. 67 (Provided by Mobley lab) NCBI: txid199310 Escherichia coli, UTI89 Mulvey et al. 68 (Provided by Mobley lab) NCBI: txid364106 Pseudomonas aeruginosa, PAK Provided by Sandkvist lab NCBI: txid1009714 Salmonella enterica serovar Typhimurium, SL1344 Provided by O’Riordan lab NCBI: txid216597 Staphylococcus aureus, USA300 LAC JE Provided by O’Riordan lab N/A Enterococcus faecalis Provided by Huffnagle lab N/A Lactobacillus johnsonii, MR-1 Provided by Huffnagle lab N/A Bacteroides thetaiotaomicron, VPI-5482 Provided by Martens lab NCBI: txid226186 Bacteroides fragilis, NCTC 9343 Provided by Martens lab NCBI: txid272559 Bacteroides ovatus, ATCC 8483 Provided by Martens lab NCBI: txid411476 Chemicals, peptides, and recombinant proteins Lipopolysaccharides from Escherichia coli O111:B4 Millipore Sigma Cat# L2630 Critical commercial assays Qiagen RNeasy mini kit Qiagen Cat# 74104 Deposited data RNAseq This paper NCBI SRA: PRJNA1290278 Metabolomics data This paper MassIVE: MSV000100081 Experimental models: Cell lines HEK-BlueTM mDectin-2 Cells Invivogen Cat# hkb-mdect2; RRID: CVCL_VV23 HEK-BlueTM mDectin-1b Cells Invivogen Cat# hkb-mdect1b; RRID: CVCL_VV21 HEK-BlueTM Null1-v Cells Invivogen Cat# hkb-null1v; RRID: CVCL_A7YL WT primary bone marrow-derived macrophages (BMDM) from Mus musculus C57BL/6J McFadden et al. 103 N/A Experimental models: Organisms/strains Candida albicans, SC5314 O’Meara et al. 44 NCBI: txid237561 (Continued on next page) Current Biology 36, 1–18.e1–e8, February 2, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Candida albicans, 813-96 Anderson et al. 22 N/A Candida albicans, 946-12 Anderson et al. 22 N/A Candida albicans, P37039 Hirakawa et al. 60 NCBI: txid1094993 Candida albicans, 824-51 Anderson et al. 22 N/A Candida albicans, 847-7 Anderson et al. 22 N/A Candida albicans, P34048 Hirakawa et al. 60 NCBI: txid1094991 Candida albicans, P57055 Hirakawa et al. 60 NCBI: txid1094996 Candida albicans, P78042 Hirakawa et al. 60 NCBI: txid1095000 Candida albicans, 811-36 Anderson et al. 22 N/A Candida albicans, P75063 Hirakawa et al. 60 NCBI: txid1094997 Candida dubliniensis, Wü 284 Provided by Righi lab N/A Candida tropicalis, ATCC 44508 Provided by Righi lab N/A Candida parapsilosis, ATCC 90018 Provided by Righi lab N/A Candida auris, AR0386 CDC antimicrobial resistance bank N/A Candida glabrata, vaginal isolate Provided by Righi lab N/A Candida albicans, SN152 Homann et al. 72 N/A Candida albicans, SN152 rlm1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 sko1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 bcrΔ/Δ Homann et al. 72 N/A Candida albicans, SN152 cup9Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 tec1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 crz1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 mig1Δ/Δ Homann et al. 72 N/A Candida albicans, SN250 hog1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 pbs2Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 mkc1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 cek1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 chk1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 nik1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 sho1Δ/Δ Noble et al. 74 N/A Candida albicans, SC5314 sln1Δ/Δ This paper N/A Candida albicans, SC5314 pENO1-iRFP Ost et al. 48 N/A Oligonucleotides Primers to construct sln1Δ/Δ mutant, see Table S1 This paper N/A Recombinant DNA Plasmid: pLC49; FLP-NAT, natR, ampR Shen et al. 104 N/A Plasmid: pLC963; CaCas9, sgRNA, ampR Veri et al. 105 N/A Software and algorithms ImageJ Schindelin et al. 106 https://imagej.net/software/fiji/; RRID: SCR_003070 FlowJo BD Biosciences https://www.flowjo.com/; RRID: SCR_008520 BD FACSDiva BD Biosciences https://www.bdbiosciences.com/en- us/products/software/instrumentsoftware/bd-facsdiva-software; RRID: SCR001456 TopSpin Bruker https://www.bruker.com/en/products- and-solutions/mr/nmr-software/ topspin.html; RRID: SCR_014227 (Continued on next page) e2 Current Biology 36, 1–18.e1–e8, February 2, 2026 Article
Recombinant:Article Title: Bacterial metabolites induce cell wall remodeling, antifungal resistance, and immune recognition of commensal fungi.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc-hDectin-1a Invivogen Cat# fc-hdec1a-2 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21445 Alexa Fluor® 647 AffiniPure® Goat Anti-Human Serum IgA, α chain specific Jackson ImmunoResearch Cat# 109-605-011; RRID: AB_2337883 Hog1 antibody Santa Cruz Bioscience Cat# sc-165978; RRID: AB_2012916 IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody LICOR Bioscience Cat# 926-32210; RRID: AB_621842 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 9215; RRID: AB_331762 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Bacterial and virus strains Escherichia coli, MC1061 Provided by Sandkvist lab NCBI: txid1211845 Escherichia coli, MG1655 Blattner et al. 66 (Provided by Mobley lab) NCBI: txid511145 Escherichia coli, CFT073 Mobley et al. 67 (Provided by Mobley lab) NCBI: txid199310 Escherichia coli, UTI89 Mulvey et al. 68 (Provided by Mobley lab) NCBI: txid364106 Pseudomonas aeruginosa, PAK Provided by Sandkvist lab NCBI: txid1009714 Salmonella enterica serovar Typhimurium, SL1344 Provided by O’Riordan lab NCBI: txid216597 Staphylococcus aureus, USA300 LAC JE Provided by O’Riordan lab N/A Enterococcus faecalis Provided by Huffnagle lab N/A Lactobacillus johnsonii, MR-1 Provided by Huffnagle lab N/A Bacteroides thetaiotaomicron, VPI-5482 Provided by Martens lab NCBI: txid226186 Bacteroides fragilis, NCTC 9343 Provided by Martens lab NCBI: txid272559 Bacteroides ovatus, ATCC 8483 Provided by Martens lab NCBI: txid411476 Chemicals, peptides, and recombinant proteins Lipopolysaccharides from Escherichia coli O111:B4 Millipore Sigma Cat# L2630 Critical commercial assays Qiagen RNeasy mini kit Qiagen Cat# 74104 Deposited data RNAseq This paper NCBI SRA: PRJNA1290278 Metabolomics data This paper MassIVE: MSV000100081 Experimental models: Cell lines HEK-BlueTM mDectin-2 Cells Invivogen Cat# hkb-mdect2; RRID: CVCL_VV23 HEK-BlueTM mDectin-1b Cells Invivogen Cat# hkb-mdect1b; RRID: CVCL_VV21 HEK-BlueTM Null1-v Cells Invivogen Cat# hkb-null1v; RRID: CVCL_A7YL WT primary bone marrow-derived macrophages (BMDM) from Mus musculus C57BL/6J McFadden et al. 103 N/A Experimental models: Organisms/strains Candida albicans, SC5314 O’Meara et al. 44 NCBI: txid237561 (Continued on next page) Current Biology 36, 1–18.e1–e8, February 2, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Candida albicans, 813-96 Anderson et al. 22 N/A Candida albicans, 946-12 Anderson et al. 22 N/A Candida albicans, P37039 Hirakawa et al. 60 NCBI: txid1094993 Candida albicans, 824-51 Anderson et al. 22 N/A Candida albicans, 847-7 Anderson et al. 22 N/A Candida albicans, P34048 Hirakawa et al. 60 NCBI: txid1094991 Candida albicans, P57055 Hirakawa et al. 60 NCBI: txid1094996 Candida albicans, P78042 Hirakawa et al. 60 NCBI: txid1095000 Candida albicans, 811-36 Anderson et al. 22 N/A Candida albicans, P75063 Hirakawa et al. 60 NCBI: txid1094997 Candida dubliniensis, Wü 284 Provided by Righi lab N/A Candida tropicalis, ATCC 44508 Provided by Righi lab N/A Candida parapsilosis, ATCC 90018 Provided by Righi lab N/A Candida auris, AR0386 CDC antimicrobial resistance bank N/A Candida glabrata, vaginal isolate Provided by Righi lab N/A Candida albicans, SN152 Homann et al. 72 N/A Candida albicans, SN152 rlm1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 sko1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 bcrΔ/Δ Homann et al. 72 N/A Candida albicans, SN152 cup9Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 tec1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 crz1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 mig1Δ/Δ Homann et al. 72 N/A Candida albicans, SN250 hog1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 pbs2Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 mkc1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 cek1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 chk1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 nik1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 sho1Δ/Δ Noble et al. 74 N/A Candida albicans, SC5314 sln1Δ/Δ This paper N/A Candida albicans, SC5314 pENO1-iRFP Ost et al. 48 N/A Oligonucleotides Primers to construct sln1Δ/Δ mutant, see Table S1 This paper N/A Recombinant DNA Plasmid: pLC49; FLP-NAT, natR, ampR Shen et al. 104 N/A Plasmid: pLC963; CaCas9, sgRNA, ampR Veri et al. 105 N/A Software and algorithms ImageJ Schindelin et al. 106 https://imagej.net/software/fiji/; RRID: SCR_003070 FlowJo BD Biosciences https://www.flowjo.com/; RRID: SCR_008520 BD FACSDiva BD Biosciences https://www.bdbiosciences.com/en- us/products/software/instrumentsoftware/bd-facsdiva-software; RRID: SCR001456 TopSpin Bruker https://www.bruker.com/en/products- and-solutions/mr/nmr-software/ topspin.html; RRID: SCR_014227 (Continued on next page) e2 Current Biology 36, 1–18.e1–e8, February 2, 2026 Article
RNA sequencing:Article Title: Bacterial metabolites induce cell wall remodeling, antifungal resistance, and immune recognition of commensal fungi.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc-hDectin-1a Invivogen Cat# fc-hdec1a-2 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21445 Alexa Fluor® 647 AffiniPure® Goat Anti-Human Serum IgA, α chain specific Jackson ImmunoResearch Cat# 109-605-011; RRID: AB_2337883 Hog1 antibody Santa Cruz Bioscience Cat# sc-165978; RRID: AB_2012916 IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody LICOR Bioscience Cat# 926-32210; RRID: AB_621842 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 9215; RRID: AB_331762 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Bacterial and virus strains Escherichia coli, MC1061 Provided by Sandkvist lab NCBI: txid1211845 Escherichia coli, MG1655 Blattner et al. 66 (Provided by Mobley lab) NCBI: txid511145 Escherichia coli, CFT073 Mobley et al. 67 (Provided by Mobley lab) NCBI: txid199310 Escherichia coli, UTI89 Mulvey et al. 68 (Provided by Mobley lab) NCBI: txid364106 Pseudomonas aeruginosa, PAK Provided by Sandkvist lab NCBI: txid1009714 Salmonella enterica serovar Typhimurium, SL1344 Provided by O’Riordan lab NCBI: txid216597 Staphylococcus aureus, USA300 LAC JE Provided by O’Riordan lab N/A Enterococcus faecalis Provided by Huffnagle lab N/A Lactobacillus johnsonii, MR-1 Provided by Huffnagle lab N/A Bacteroides thetaiotaomicron, VPI-5482 Provided by Martens lab NCBI: txid226186 Bacteroides fragilis, NCTC 9343 Provided by Martens lab NCBI: txid272559 Bacteroides ovatus, ATCC 8483 Provided by Martens lab NCBI: txid411476 Chemicals, peptides, and recombinant proteins Lipopolysaccharides from Escherichia coli O111:B4 Millipore Sigma Cat# L2630 Critical commercial assays Qiagen RNeasy mini kit Qiagen Cat# 74104 Deposited data RNAseq This paper NCBI SRA: PRJNA1290278 Metabolomics data This paper MassIVE: MSV000100081 Experimental models: Cell lines HEK-BlueTM mDectin-2 Cells Invivogen Cat# hkb-mdect2; RRID: CVCL_VV23 HEK-BlueTM mDectin-1b Cells Invivogen Cat# hkb-mdect1b; RRID: CVCL_VV21 HEK-BlueTM Null1-v Cells Invivogen Cat# hkb-null1v; RRID: CVCL_A7YL WT primary bone marrow-derived macrophages (BMDM) from Mus musculus C57BL/6J McFadden et al. 103 N/A Experimental models: Organisms/strains Candida albicans, SC5314 O’Meara et al. 44 NCBI: txid237561 (Continued on next page) Current Biology 36, 1–18.e1–e8, February 2, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Candida albicans, 813-96 Anderson et al. 22 N/A Candida albicans, 946-12 Anderson et al. 22 N/A Candida albicans, P37039 Hirakawa et al. 60 NCBI: txid1094993 Candida albicans, 824-51 Anderson et al. 22 N/A Candida albicans, 847-7 Anderson et al. 22 N/A Candida albicans, P34048 Hirakawa et al. 60 NCBI: txid1094991 Candida albicans, P57055 Hirakawa et al. 60 NCBI: txid1094996 Candida albicans, P78042 Hirakawa et al. 60 NCBI: txid1095000 Candida albicans, 811-36 Anderson et al. 22 N/A Candida albicans, P75063 Hirakawa et al. 60 NCBI: txid1094997 Candida dubliniensis, Wü 284 Provided by Righi lab N/A Candida tropicalis, ATCC 44508 Provided by Righi lab N/A Candida parapsilosis, ATCC 90018 Provided by Righi lab N/A Candida auris, AR0386 CDC antimicrobial resistance bank N/A Candida glabrata, vaginal isolate Provided by Righi lab N/A Candida albicans, SN152 Homann et al. 72 N/A Candida albicans, SN152 rlm1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 sko1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 bcrΔ/Δ Homann et al. 72 N/A Candida albicans, SN152 cup9Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 tec1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 crz1Δ/Δ Homann et al. 72 N/A Candida albicans, SN152 mig1Δ/Δ Homann et al. 72 N/A Candida albicans, SN250 hog1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 pbs2Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 mkc1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 cek1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 chk1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 nik1Δ/Δ Noble et al. 74 N/A Candida albicans, SN250 sho1Δ/Δ Noble et al. 74 N/A Candida albicans, SC5314 sln1Δ/Δ This paper N/A Candida albicans, SC5314 pENO1-iRFP Ost et al. 48 N/A Oligonucleotides Primers to construct sln1Δ/Δ mutant, see Table S1 This paper N/A Recombinant DNA Plasmid: pLC49; FLP-NAT, natR, ampR Shen et al. 104 N/A Plasmid: pLC963; CaCas9, sgRNA, ampR Veri et al. 105 N/A Software and algorithms ImageJ Schindelin et al. 106 https://imagej.net/software/fiji/; RRID: SCR_003070 FlowJo BD Biosciences https://www.flowjo.com/; RRID: SCR_008520 BD FACSDiva BD Biosciences https://www.bdbiosciences.com/en- us/products/software/instrumentsoftware/bd-facsdiva-software; RRID: SCR001456 TopSpin Bruker https://www.bruker.com/en/products- and-solutions/mr/nmr-software/ topspin.html; RRID: SCR_014227 (Continued on next page) e2 Current Biology 36, 1–18.e1–e8, February 2, 2026 Article
|
InvivoGen is a verified supplier 
InvivoGen manufactures this product 